Description
Intended Uses: This sandwich kit is for the accurate quantitative detection of Rat Bcl2/Adenovirus E1B 19kDa Interacting Protein 3 (also known as BNIP3) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rat BNIP3 antibody. BNIP3 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat BNIP3 Antibody is added and binds to BNIP3 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated BNIP3 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat BNIP3. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm